Application

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

This Anti-CD19 antibody (human), PE, clone SJ25C1 is validated for use in Flow Cytometry for the detection of CD19.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The SJ25C1 antibody reacts with human CD19, a 95 kDa glycoprotein which acts as a co-receptor, along with CD21 (CR2), CD81 (TAPA-1) and CD225 (Leu13), in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounter. Upon activation and tyrosine phosphorylation, the CD19 molecule can provide an anchor for cytoplasmic signaling proteins such as GRB2, SOS or PLCG2. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, through development, and is finally lost upon transition to mature plasma cells.The SJ25C1 antibody is widely used as a phenotypic marker for CD19 expression on B cells, as well as on dendritic cell subsets.

Immunogen

Immunogen information for this antibody is proprietary information.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified Mouse monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.

Purified by size-exclusion chromatography

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg from a representative lot detected CD19 in one million human peripheral blood lymphocytes.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.